General Information

  • ID:  hor004895
  • Uniprot ID:  P0DL76
  • Protein name:  Conopressin-T
  • Gene name:  NA
  • Organism:  Conus tulipa (Fish-hunting cone snail) (Tulip cone)
  • Family:  Vasopressin/oxytocin family
  • Source:  animal
  • Expression:  Expressed by the venom duct.
  • Disease:  NA
  • Comments:  NA
  • Taxonomy:  Gastridium (subgenus), Conus (genus), Conidae (family), Conoidea (superfamily), Neogastropoda (order), Caenogastropoda (subclass), Gastropoda (class), Mollusca (phylum), Lophotrochozoa, Spiralia, Protostomia, Bilateria, Eumetazoa, Metazoa (kingdom), Opisthokonta, Eukaryota (superkingdom), cellular organisms
  • GO MF:  GO:0090729 toxin activity
  • GO BP:  GO:0035821 modulation of process of another organism
  • GO CC:  GO:0005576 extracellular region

Sequence Information

  • Sequence:  CYIQNCLRV
  • Length:  9
  • Propeptide:  CYIQNCLRV
  • Signal peptide:  NA
  • Modification:  T9 Valine amide
  • Glycosylation:  NA
  • Mutagenesis:  7-7L->P: 8-fold increase in affinity for V1aR/AVPR1A, no change in affinity for V1b and oxytocin receptors (OXTR) and gain of affinity for V2R (Ki=1.8 uM).

Activity

  • Function:  Interacts with high affinity with oxytocin receptor (OXTR) (Ki=108 nM) and vasopressin V1a receptor (V1aR/AVPR1A) (Ki=319 nM). Acts as a partial agonist on OXTR (Kact=37 nM, 22% of oxytocin maximum activity at 10 ¦ÌM), and as a full antagonist on V1aR (Kinact=329 nM).Does not show binding with vasopressin V1b and V2 receptors (up to 10 ¦ÌM). Shows a weak agonist activity on V1bR/AVPR1B (9% at 10 ¦ÌM).Position 9 is important residue for antagonist activity on V1aR/AVPR1A (a Gly in this position produces agonist activi
  • Mechanism:  Negative results: does not show binding with vasopressin V1b and V2 receptors (up to 10 uM). Shows a weak agonist activity on V1bR/AVPR1B (9% at 10 uM).
  • Cross BBB:  NA
  • Target:  NA
  • Target Unid:  NA
  • IC50: NA
  • EC50: NA
  • ED50: NA
  • kd: NA
  • Half life: NA

Structure

  • Disulfide bond:  1-6
  • Structure ID:  NA

  • Structure: (PDB_ID-from https://www.rcsb.org/; AlphaFold_DB_ID-from https://alphafold.ebi.ac.uk/; hordbxxxxxx_AF2.pdb was predicted structure by AlphaFold2; hordbxxxxxx_ESM.pdb was predicted structure by ESMFold)
    •    hor004895_AF2.pdbhor004895_ESM.pdb

Physical Information

Mass: 125423 Formula: C47H78N14O13S2
Absent amino acids: ADEFGHKMPSTW Common amino acids: C
pI: 8.22 Basic residues: 1
Polar residues: 4 Hydrophobic residues: 3
Hydrophobicity: 52.22 Boman Index: -1080
Half-Life: 1.2 hour Half-Life Yeast: >20 hour
Half-Life E.Coli: >10 hour Aliphatic Index 118.89
Instability Index: 4848.89 Extinction Coefficient cystines: 1615
Absorbance 280nm: 201.88

Literature

  • PubMed ID:  18174156
  • Title:  Conopressin-T from Conus tulipa reveals an antagonist switch in vasopressin-like peptides.